BBa_I714945

BBa_I714945 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I714945
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xili Liu
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:07:49

pLX012



Types
DnaRegion

Roles
Plasmid

plasmid

Sequences BBa_I714945_sequence (Version 1)

Description

a plasmid with EGFP reporter to report double repressed promoter activity

Notes

EGFP with strong SD signal, a MCS before EGFP to insert double repressed promoter, high efficient double terminator T1T2 locates on both upstream of MCS and downstream of EGFP.

Source

pCRT-1

Sequence Annotation Location Component / Role(s)
T1T2
SDY
start
EGFP
stop
T1T2
Amp
MCS
ColE ori
1,203
248,262
263,263
263,967
967,967
1028,1230
2264,3195
229,246
1494,2082
feature/BioBrick engineered_region
ribosome_entry_site feature/rbs
start_codon feature/start
CDS feature/protein
stop_codon feature/stop
feature/BioBrick engineered_region
feature/protein CDS
sequence_feature feature/dna
feature/conserved conserved_region
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I714945/1