BBa_I716016

BBa_I716016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Yu
Date created: 2007-07-23 11:00:00
Date modified: 2015-08-31 04:07:50

(yfbE-rbs-RFP) jp paper style



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_I716016_sequence (Version 1)

    Description

    iron promoter yfbE with RBS and ATG on it, with an RFP without ATG added to the end

    Notes


    
make (yfbE-rbs-ATG) + (RFP_no_ATG) from pcr prod
    

    
PCR product (RFP_no_ATG), digested BglII/XhoI
    
Digest I716013 (yfbE-rbs-ATF) BamHI/XhoI (2387+9), L
    
Ligate, get I716016 (yfbE-rbs-RFP_jp)
    

    

    

    
or for rfp step, (I716015)  2063+693 get small.

    {{BerkiGEM2007-Biobrick2.0Intro}}

    Source

    PCR product RFP_no_ATG + I716013

    igem#experience
    Issues
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716016/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (d02b3767)