BBa_I716302

BBa_I716302 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716302

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: vincent parker

Date created: 2007-06-26 11:00:00

Date modified: 2015-08-31 04:07:52

ots B

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_I716302_sequence (Version 1)

Description

otsB

Notes

{{BERKiGEM2007-Biobrick2.0Intro}}

Source

PCR otsBF/otsBIR on otsB (485 bp, gp=A)
PCR otsBIF/otsBR on otsB (381 bp, gp=B)

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PCR otsBF/otsBR on A+B (832 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI, 2054+910, L)
Product is pBca9145-BBa I716302

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otsBF Forward BglII site anneals to otsB cgtaaAGATCTATGACAGAACCGTTAACCGA
otsBIF Removes internal BglII site GCAACGTATTACTCAGATtTGGCCACAAATGGCG
otsBIR Removes internal BglII site CGCCATTTGTGGCCAaATCTGAGTAATACGTTGC
otsBR Reverse XhoI site anneals to otsB cgtatCTCGAGttaGGATCCTTAGATACTACGACTAAACG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_I716302/1

Member of these Collections

• iGEM Parts Registry