BBa_I716332

BBa_I716332 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716332

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: nhu nguyen

Date created: 2007-07-10 11:00:00

Date modified: 2015-08-31 04:07:52

ThpB

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_I716332_sequence (Version 1)

Description

This gene is very similar to OtsA and OtsB, and this will help prevent dehydration.

Notes

((BerkiGEM2007-Biobrick2.0Intro))

Source

ThpB
From IGEM07
PCR ThpBF/ ThpBIR on ThpB (610 bp, gp=A)
PCR ThpBR/ ThpBIF on ThpB (712 bp, gp=B)

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PCR ThpBAF/ThpBAR on A+B (1285bp BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
Product pBca9145-BBa I716332

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ThpBF Forward BglII site anneals to ThpB ctagaAGATCTgtgaccatcaccccgcccgc
ThpBIF Removing XhoI site in ThpB ggttcgagcggctcctTgaggaccagggctccggac
ThpBIR Removing XhoI site in ThpD gtccggagccctggtcctcAaggagccgctcgaacc
ThpBR Reverse XhoI site anneals to ThpB cgtcactcgagtcaggccgtttcgcggacgc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716332/1

Member of these Collections

• iGEM Parts Registry