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BBa_I718006

BBa_I718006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I718006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Bikard

Date created: 2007-10-18 11:00:00

Date modified: 2015-08-31 04:07:52

pTet-lox71-gfp-terminator-lox66-mRFP

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_I718006_sequence (Version 1)

Description

lox71-gfp-ter-lox66 are cloned in the plasmid J61002 between the ptet promoter (BBa_R0040) and the mRFP1 reporter gene.

The construct is (including standard restriction sites):
(EcoRI,XbaI)-pTet-lox71-gfp-terminator-lox66-(SpeI)-mRFP1-(PstI)

In this construction, any biobrick can be cloned as a backward insert instead of the mRFP gene.

Notes

none

Source

none

Components

Access	Instance	Definition
public public public public public public public	BBa_R0040 BBa_J37028 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012 BBa_J37026	p(tetR) BBa_J37028 BBa_B0030 GFP BBa_B0010 BBa_B0012 BBa_J37026

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_J37028 BBa_B0030 BBa_E0040 BBa_B0010 BBa_B0012 BBa_J37026	1,54 63,96 105,119 126,845 854,933 942,982 991,1024	p(tetR) BBa_J37028 BBa_B0030 GFP BBa_B0010 BBa_B0012 BBa_J37026