BBa_I728004

BBa_I728004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I728004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: aditya kohli
Date created: 2007-06-20 11:00:00
Date modified: 2015-08-31 04:07:55

CPX with polystyrene-binding peptide



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_I728004_sequence (Version 1)

Description

CPX expresses the polystyrene binding peptide on the outside of the E.Coli cell. We have added in a His tag, a trypsin cleavage site and we have flanked this sequence with Ecor1 and Xba1 cut sites.

Notes

None.

Source

Comes from Ompx protein.

Sequence Annotation Location Component / Role(s)
xBa1
EcoR1
Native Signal Sequence
Linker w/ sfi1 cut site
His Tag
Trypsin Cleavage
Polystyrene Binding Peptide
Linker w/sfi cut site
S54-F148
N-C Terminus Linker
A1-S53
1,6
628,633
7,75
76,90
91,108
109,114
115,153
154,171
172,456
457,468
469,627
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/tag tag
feature/misc sequence_feature
feature/protein CDS
sequence_feature feature/misc
CDS feature/protein
feature/misc sequence_feature
CDS feature/protein
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I728004/1