Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhan Jian
Date created: 2007-10-25 11:00:00
Date modified: 2015-08-31 04:08:00
Ptemplate1 + RBS.ARL2A0103
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_I732952_sequence (Version 1) |
Description
{| border="1"|-
|align="center"|Genotype
|align="center"|Character
|align="center"|Name
|-
|Top10/pT-TERM
|So not express any repressors
|align="center"|CR00
|-
|Top10/pT-ARL4A0604
|Constitutively express ARL4A0604
|align="center"|CR01
|-
|Top10/pT-ARL2A0203
|Constitutively express ARL2A0203
|align="center"|CR10
|-
|Top10/pTet-ARL4A0604-ARL2A203
|Constitutively express ARL4A0604 and ARL2A0203
|align="center"|CR11
|}
Notes
N/ASource
N/A| Sequence Annotation | Location | Component / Role(s) |
| pBR322 origin beta-lactamase (ampicillin resistance marker) beta-lactamase (ampR) promoter EcoRI BioBrick suffix XbaI Verification forward (VF2) primer binding site Stem loop End of terminator E. coli his operon terminator Stem loop Deletion of AC Verification reverse (VR) primer binding site Ptemplate1? | 261,880 1035,1895 1937,1965 2136,2141 1,21 2301,2306 2018,2037 2107,2126 2094,2097 22,93 30,64 34,35 157,176 2142,2300 | feature/dna sequence_feature CDS feature/cds promoter feature/promoter feature/dna sequence_feature sequence_feature feature/dna sequence_feature feature/dna primer_binding_site feature/primer_binding feature/stem_loop stem_loop feature/stem_loop stem_loop feature/stem_loop stem_loop feature/stem_loop stem_loop sequence_alteration feature/mutation primer_binding_site feature/primer_binding promoter feature/promoter |