BBa_I737012

BBa_I737012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I737012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dave Curran
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:08:01

With AHL, light -> area of no GFP.



Types
DnaRegion

Roles
Project

engineered_region

Sequences BBa_I737012_sequence (Version 1)

Description

There was some amniguity on the registry site about which parts were OmpC, and which were OmpF. The two have opposing functionality, so this was an important distinction. Looking at the literature, there was also some question as to which promoter we would be able to use in our system, though we wanted to use OmpF. This is the reversal of the logic circuit of the 2007 University of Calgary project built with OmpC, and so requires the light sensor (M30109) to function. After addition of AHL to the media, the cells will be responsive to 650 nm light, and begin making GFP. When they sense light, they will stop expressing GFP (E0040), and will remain inactive until any AHL in the media breaks down. In the absence of either AHL, the bacteria should do nothing.

Notes

The protein LuxR is present in the system twice; it should be expressed first upon exposure to light, and should then activate expression of yet more LuxR. This allows continued expression of GFP even when the light is removed.

Source

This part was made using existing biobrick parts.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I737012/1