LIMS1

BBa_I742110 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742110

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: sarah hollingshead

Date created: 2007-10-18 11:00:00

Date modified: 2015-08-31 04:08:02

LIMS1

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_I742110_sequence (Version 1)

Description

Limonene synthase 1 from Citrus limon (accession AF514287)
Synthesised by Geneart.

Limonene synthase converts farnesyl-diphosphate to + limonene, which is a component of lemon scent.

Notes

several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG

Source

Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002)

Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990)

Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
(+) limonene synthase coding region	1,1671	feature/cds, CDS
start	1,3	feature/start, start_codon
stop	1666,1671	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742110/1

Member of these Collections

• iGEM Parts Registry