Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Dahl
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:02
Reverse complement Lac promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I742124_sequence (Version 1) |
Description
This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives.The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites).
Notes
When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order.Source
This sequence is part of the E.coli K12 genome.| Sequence Annotation | Location | Component / Role(s) |
| CAP binding site LacI binding site Plac -35 Plac -10 | 78,115 11,31 61,66 38,43 | operator feature/operator feature/operator operator feature/promoter promoter promoter feature/promoter |