BBa_I742125

BBa_I742125 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742125

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caroline Dahl

Date created: 2007-10-21 11:00:00

Date modified: 2015-08-31 04:08:02

recombination site + Reporter (YFP)

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_I742125_sequence (Version 1)

Description

This brick contains an inverted dif-site and the YFP reporter (BBa_E0430). If combined with a down- or upstream dif site in the same orientation, the sequence between the dif-sites will excise during bacterial cell division (in this case YFP is excised). If combined with a dif site pointing in the opposite direction, the flanked sequence will invert.

Notes

See individual parts for details.

Source

The dif-site is an innate component of the E. coli K12 genome. YFP is BBa_E0430.

Components

Access	Instance	Definition
public	BBa_I742102	difR
public	BBa_B0034	BBa_B0034
public	BBa_E0030	eyfp
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I742102	1,31	difR
BBa_B0034	40,51	BBa_B0034
BBa_E0030	58,780	eyfp
BBa_B0010	789,868	BBa_B0010
BBa_B0012	877,917	BBa_B0012

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742125/1

Member of these Collections

• iGEM Parts Registry