Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
Lycopene (red pigment) producing operon without PstI sites
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I742136_sequence (Version 1) |
Description
Lycopene is synthesised by three proteins encoded by the three genes CrtE, CrtB and CrtI.The three enzymes involved act sequentially on farnesyl diphosphate, to produce lycopene.
Lycopene has a red pigment and acts as a powerful antioxident in the body.
More information may be found on the Edinburgh Yoghurt Wiki
Notes
The two PstI sites within CrtI have been mutated out using point mutagenesis.All three genes were cloned so they still retain their native ribosome binding sites.
Source
All three genes in this pathway were isolated from Pantoea ananatis (formerly Erwinia uredovora)DSMZ 30080, ATCC 19321
Information about the carotenoid biosynthesis pathways may be found in the following references:
Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)
Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
| Access | Instance | Definition |
| public public | BBa_I742116 BBa_I742133 | BBa_I742116 BBa_I742133 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I742116 BBa_I742133 | 1,954 963,3392 | BBa_I742116 BBa_I742133 |