| Types | DnaRegion
|
| Roles | Plasmid
plasmid
|
| Sequences | BBa_I745000_sequence (Version 1)
|
Description
This backbone consists of the TRE promoter, activated when the reverse transactivator, rtTA, and Doxycycline together bind the promoter. An IRES (internal ribosomal binding site) follows the TRE promoter and EGFP follows the IRES.
Notes
We wanted to create a vector that could be modular and one that would allow one to use any promoter, any gene of interest and any selection/fluorescent marker. We designed the promoter with flanking PacI and EcoRI sites and the gene site with SfiI, EcoRI, BamHI and NheI sites. However, due to the lack of available sites in a lentivector, we had difficulty designing a modular IRES-indicator/selection fragment. Work is underway to correct this.
Source
This plasmid was designed by the Weiss lab for use as a lentiviral vector.
