BBa_I746000

BBa_I746000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I746000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Wyatt
Date created: 2007-10-19 11:00:00
Date modified: 2015-08-31 04:08:04

AIP generator (agrB + agrD with RBSes)



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_I746000_sequence (Version 1)

Description

This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBirck-compatible signalling mechanism.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.

This part contains the coding regions for group I agrB and agrD (in that order), prefixed by the B0034 RBS and separated by a SaII site.

Notes

N/A

Source

The original sequences for agrB and agrD were taken from the S Aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.

Sequence Annotation Location Component / Role(s)
B0034-sequence RBS
agrB coding sequence
B0034-sequence RBS
SaII site
agrD coding sequence
1,12
19,591
598,609
592,597
616,759
ribosome_entry_site feature/rbs
feature/cds CDS
feature/rbs ribosome_entry_site
sequence_feature feature/misc
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I746000/1