BBa_I746203

BBa_I746203 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I746203
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Narin Hengrung
Date created: 2007-10-20 11:00:00
Date modified: 2015-08-31 04:08:04

Intermediate for Outer Membrane Permeability Device



Types
DnaRegion

Roles
engineered_region

Intermediate

Sequences BBa_I746203_sequence (Version 1)

Description

This part contains the coding region for the L8T mutant of FepA. When expressed, this protein forms an open diffusion channel in the outer membrane of E. coli, allowing molecules of at least 1400Da to pass through. I746201 and I746202 both are devices to express this protein at different levels. This part does not have a promotor, to allow new constructs to easily be made.

Notes

Removal of an EcoR1 site by site directed mutagenesis.

Source

The L8T FepA mutant was kindly supplied to us in a plasmid by Professor P.E. Klebba.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I746203/1