BBa_I756034

BBa_I756034 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I756034
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Lei
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:08:07

CD18-gsl-Nub



    • Details

    Types
    DnaRegion

    Roles
    Signalling

    engineered_region

    Sequences BBa_I756034_sequence (Version 1)

    Description

    CD18-gsl-Nub

    CD18 is the second half of the LFA1 signaling dimer which binds to ICAM. To induce an output from ligand mediated signaling, we used the split ubiquitin system. We designed LFA1 dimerisation to produce the same output as our other signaling receptors - mLexA-VP16.

    Notes

    none

    Source

    kpn1 (1-6bp)

    kozak (7-12bp)

    CD18 from Integrin beata2-mYFP, Kim, M et al. Science 2003, 5640:1720-5 (13-2319bp)

    BspQ1 linker (2320-2361bp)

    NubG assembled from oligos (2362-2490bp)

    stop stop (2491-2496bp)

    xba1 (2497-2502bp)

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I756034/1
     

    Attachments

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