Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Chou
Date created: 2007-08-15 11:00:00
Date modified: 2015-08-31 04:08:10
YopH-EE under weak constitutive promotor
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_I766676_sequence (Version 1) |
Description
This device will express YopH, a pathogen derived irreversible inhibitor (phosphotase) of MapK Fus3 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.Notes
Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.Source
conposite part| Access | Instance | Definition |
| public public public public public | BBa_I766557 BBa_I766007 BBa_I766101 BBa_I766102 BBa_I766600 | BBa_I766557 BBa_I766007 BBa_I766101 BBa_I766102 BBa_I766600 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I766557 BBa_I766007 BBa_I766101 BBa_I766102 BBa_I766600 | 1,601 610,2022 2029,2748 2757,2918 2927,3144 | BBa_I766557 BBa_I766007 BBa_I766101 BBa_I766102 BBa_I766600 |