BBa_I769102

BBa_I769102 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I769102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mackenize Cowell

Date created: 2007-06-26 11:00:00

Date modified: 2015-08-31 04:08:10

Feruloyl-CoA hydratase for vanillin biosynthesis

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_I769102_sequence (Version 1)

Description

One of two steps in the bioconversion of ferulic acid to vanillin. The enzyme is from Pseudomonas fluorescens and it does work with E. coli. It requires both ech and fcs for vanillin biosynthesis.

Notes

Changed stop codon to TAATAA

Source

Genbank accession: AJ536325; Microb Cell Fact. 2007; 6: 13.
Published online 2007 April 16. doi: 10.1186/1475-2859-6-13.
Copyright ?? 2007 Barghini et al; licensee BioMed Central Ltd.
Vanillin production using metabolically engineered Escherichia coli under non-growing conditions
Paolo Barghini,1 Diana Di Gioia,2 Fabio Fava,2 and Maurizio Ruzzi corresponding author1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start codon	1,3	feature/start, start_codon
Stop codon	829,834	stop_codon, feature/stop
Feruloyl-CoA hydratase (ech)	1,834	feature/cds, CDS

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I769102/1

Member of these Collections

• iGEM Parts Registry