BBa_J07012

BBa_J07012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J07012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: mit_igem_2005

Date created: 2005-07-07 11:00:00

Date modified: 2015-08-31 04:08:19

scFv anti-fluorescein [4-4-20]

Details

• Types: DnaRegion
• Roles: engineered_region; Signalling
• Sequences: BBa_J07012_sequence (Version 1)

Description

single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the weakest of the three specified sequences.

DNA insert in the pCT-4-4-20 (also known as pcT-302) from NheI to XhoI

Source

Substantial Energetic Improvement with Minimal Structural Perturbation in a High Affinity Mutant Antibody

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CDR1 VL	61,120	feature/misc, sequence_feature
CDR2 VL	163,183	sequence_feature, feature/misc
CDR3 VL	280,306	sequence_feature, feature/misc
Linker	337,411	sequence_feature, feature/misc
CDR1 VH	502,513	feature/misc, sequence_feature
CDR2 VH	559,615	feature/misc, sequence_feature
CDR3 VH	711,732	feature/misc, sequence_feature
VL	1,336	feature/misc, sequence_feature
VH	412,762	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J07012/1

Member of these Collections

• //function/cellsignalling
• iGEM Parts Registry