BBa_J100068

BBa_J100068 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100068

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Meredith Nakano

Date created: 2012-06-26 11:00:00

Date modified: 2015-08-31 04:08:22

fadB promoter (short sequence)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_J100068_sequence (Version 1)

Description

This short sequence of the FadB promoter is repressed by phosphorylated ArcA which is part of the ArcAB system in E. coli. It is activated by Fis. ArcA and Fis have overlapping binding sites. Unlike the long version of this promoter, the short sequence is not activated by FadR since it is missing the FadR binding site. http://mic.sgmjournals.org/content/152/8/2207.full.pdf+html

Notes

This part was designed without the activation binding site of FadR so that the promoter may be repressed more readily.

Source

From E. coli and assembled by oligos.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	51,51	feature/misc, sequence_feature
+1	61,61	feature/misc, sequence_feature
-35	26,26	sequence_feature, feature/misc
ArcA	25,40	non_covalent_binding_site, feature/binding
Fis	25,44	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100068/1

Member of these Collections

• iGEM Parts Registry