BBa_J100074

BBa_J100074 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100074
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Betsy Gammon
Date created: 2012-07-04 11:00:00
Date modified: 2015-08-31 04:08:22

Long pLux Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J100074_sequence (Version 1)

Description

This is the long form of the pLux promoter. This promoter promotes the luxR gene but only in the presence of 30C6. The shorter form of the pLux promoter does not include an arcA binding site. Phosphorylated arcA typically represses this long promoter in anaerobic conditions.

Notes

This part includes 2 ArcA binding sites as well as a CRP site. It starts 238 bp upstream of the transcription factor.

Source

E. coli

Sequence Annotation Location Component / Role(s)
ArcA binding site 2
arcA binding site 1
CRP binding site
LuxR binding site
-10
+1
94,109
127,144
84,109
145,165
188,188
197,197
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
sequence_feature feature/misc
feature/misc sequence_feature
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100074/1