Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: jessica gronniger
Date created: 2013-07-16 11:00:00
Date modified: 2015-08-31 04:08:23
AdhE gene cloned into J100112
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_J100116_sequence (Version 1) |
Description
AdhE gene (J100109) was ligated between junctions C and D on scaffold (J119301). Golden Gate assembly was used to put adhE into the gap between junctions C and D. iPCR was used to generate the receiving plasmid.Notes
No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100116.Source
All parts taken from Registry.| Sequence Annotation | Location | Component / Role(s) |
| Junction A P5 (J119031) Junction B C. dog BD24 (J119028) Junction C adhE (J100109) Junction D | 1,17 18,54 55,72 73,151 152,169 170,2862 2863,2881 | non_covalent_binding_site feature/binding promoter feature/promoter feature/binding non_covalent_binding_site ribosome_entry_site feature/rbs non_covalent_binding_site feature/binding sequence_feature feature/dna feature/binding non_covalent_binding_site |