Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Prudencio
Date created: 2015-06-01 11:00:00
Date modified: 2015-08-31 04:08:24
tCloneRed with RS4 Transcriptional Riboswitch
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J100210_sequence (Version 1) |
Description
Using Golden Gate Assembly, the transcriptional riboswitch RS4 (from Wachsmuth, et al.) was ligated into the destination vector tClone Red (J119361) in place of the insert (between BsaI sites). This riboswitch is controlled by theophylline, allowing transcription if theophylline is present. However, it also has shown increased transcription levels in the presence of caffeine.Notes
Appropriate sticky ends had to be added to the RS4 sequence in order to anneal with those created by digestion of the destination vector with BsaI.Source
The destination vector is part J119361. The sequence of the riboswitch comes from the paper "De novo design of a synthetic riboswitch that regulates transcription termination," by Wachsmuth, et al. (referred to as "RS4" in that paper; doi:10.1093/nar/gks1330).| Sequence Annotation | Location | Component / Role(s) |
| P5 Promoter RS4 Transcription Riboswitch BD18 C dog RBS RFP | 1,36 41,117 122,206 207,887 | promoter feature/promoter stem_loop feature/stem_loop ribosome_entry_site feature/rbs feature/cds CDS |