Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Preziosi
Date created: 2015-06-21 11:00:00
Date modified: 2015-08-31 04:08:24
tCloneTet+Red fusion protein reporter with Riboswitch 3Shift
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_J100213_sequence (Version 1) |
Description
This is a hybrid taken from part J119386, with everything between the BsaI restriction sites removed, and a riboswitch put in its place. The riboswitch was taken from the paper "De novo design of a synthetic riboswitch that regulates transcription termination" by Wachsmuth et al. 2013. This transcriptional riboswitch is expected to be able to regulate the reporter gene (in this case, the fusion protein Tet+RFP) which follows it. By binding to the ligand, theophylline, the riboswitch will be "on," and the gene will be expressed. The absence of theophylline should turn the switch "off" and the gene won't be expressed.Notes
The plasmid that this part is located in is pUC-BR, a plasmid modified from pUC-IDT-Amp with an errant BsaI site in the Ampicillin resistance gene removed.Source
Part BBa_J119386, and Wachsmuth et al. 2013, available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575828/ . This is the same riboswitch as is included in part J100208.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Riboswitch 3 Shift BD18 C dog RBS TetA Linker sequence RFP | 1,36 41,140 145,229 230,1423 1424,1471 1472,2152 | feature/promoter promoter feature/stem_loop stem_loop feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS feature/cds CDS |