Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuk Hang Li
Date created: 2016-05-15 11:00:00
Date modified: 2016-07-12 12:31:18
B Nitrate Biosensor (both)
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J100258_sequence (Version 1) |
Description
This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by disrupting both the left and right Fis binding site, where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promtoer is inserted in to pClone Red (J119137). Nitrate induces the promoter, so RFP is expressed when nitrate is present.Notes
There is a deletion at BD18 bicistron RBS.Source
E. coli K-12 MG1655 Transcription Unit: narGHJI| Sequence Annotation | Location | Component / Role(s) |
| GFP Reverse fixed both narG promoter RFP BD18 bicistron RBS | 1,720 755,1021 1119,1800 1033,1118 | feature/cds CDS feature/promoter promoter feature/cds CDS feature/rbs ribosome_entry_site |