Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuk Hang Li
Date created: 2016-06-07 11:00:00
Date modified: 2016-07-12 12:34:16
DB Nitrate Biosensor
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J100260_sequence (Version 1) |
Description
This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by trimming the left Fis binding site and disrupting right Fis binding site, where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promoter is inserted in to pClone Red (J119137). Nitrate induces the promoter, so RFP is expressed when nitrate is present.Notes
/Source
NarGHJI operon| Sequence Annotation | Location | Component / Role(s) |
| GFP Reverse DB narG promoter RFP BD18 bicistron RBS | 1,720 755,864 962,1642 877,961 | CDS feature/cds feature/promoter promoter feature/cds CDS ribosome_entry_site feature/rbs |