BBa_J100293

BBa_J100293 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100293
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuk Hang Li
Date created: 2016-07-12 11:00:00
Date modified: 2016-07-13 03:05:53

B Biosensor + NarX



Types
DnaRegion

Roles
engineered_region

Measurement

Sequences BBa_J100293_sequence (Version 1)

Description

This construct is built by modifying B Nitrate Biosensor (J100258), which is built by inserting a modified narG promoter sequence into pClone Red (J119137) through GGA. There are binding sites for activators NarL on the narG promoter. NarX, the sensor kinase, is known to phosphorylate NarL, which is the response regulator, so we inserted the NarX gene into the plasmid replacing the reverse GFP, hoping that will increase NarL binding on the promoter. Also sensor kinase mRNAs are synthesized efficiently but they rapidly degrade. Therefore we inserted a 5' terminal stemloop upstream of the NarX gene to reduce mRNAs degradation by RNase E. In conclusion, we removed the GFP reverse and inserted a complex with a transcription terminator, a promoter, a stemloop, a RBS, and the NarX gene. The goal is that nitrate induces the promoter, then RFP is expressed when nitrate is present. However, this promoter does not induce RFP production when nitrate is present.

Notes

/

Source

narGHJI operon

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100293/1