Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-07-20 11:00:00
Date modified: 2015-08-31 04:08:26
BsaI-GFP2-BsmBI
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119002_sequence (Version 1) |
Description
This is the second half of the GFP gene split for use in solving HPP (Hamiltonian Path Problems). It is the necessary complement to GFP-1 part name: BBa_J119001It will function as a reporter if both genes are transcribed in proper order after re-assembly in E Coli.
It is also intended to be mixed with other gene halves to attempt a multi-node HPP problem that will show correct completion if all reporters are present (resistance, fluorescence, etc.). All groupings must have both halves of a specific reporter gene to function properly.
Here is the design of the forward and reverse sequences minus the entire gene sequence.
GFP_2_For (36mer)
GCAT GAATTC GGTCTC A ACCC TTGTTAATAGAATCG
4 EcoRI BsaI a 1234 15mer of GFP_2
GFP_2_Rev (158mer)
ATGC CTGCAG CGTCTC A CCAC GTGCTCAGTATCTCTATCACTGATAGGGATGTCAATCTCTATCACTGATAGGGA TTGC
4 PstI BsmBI a 1234 pTet Reverse Complement 4
(1/2 edge R.C.)
Notes
There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.Source
PCR Amplification of GFP gene template split at the sequence ACCC at 351 nt from start.| Sequence Annotation | Location | Component / Role(s) |
| EcoRI pTet R0040 | 1,6 387,440 | sequence_feature feature/misc promoter feature/promoter |