BBa_J119003

BBa_J119003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caleb J. Carr

Date created: 2011-07-20 11:00:00

Date modified: 2015-08-31 04:08:26

BsmBI-Rbs-LacZ1-BsaI

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J119003_sequence (Version 1)

Description

First half of the LacZ Gene (LacZ-1), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119004, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli Jm109. It functions as a reporter gene when properly assembled with LacZ-2 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly.

Beta-Gal_1_For (58mer)
5??? GCAT GAATTC CGTCTC A GTGG CA AAAGAGGAGAAA CGTACGAC ATGACCATGATTACG 3???

(4nt , EcoRI, BsmBI , a , Halfedge1234, nn, RBS 0034 , 8 , 15mer of Beta-Gal)

Beta-Gal_1_Rev (36mer)

5???ATGC CTGCAG GGTCTC A CAGG TAGCGAAAGCCATTT 3???)

(4nt, PstI , BsaI , a , Gene1234RC , 15mer of Beta-Gal reverse

Notes

This is NOT a BioBrick part. It is only functional if properly re-assembled with J11904. Can be used in combination with other split-genes to solve HPP (Hamiltonian Path Problems).

Source

PCR amplification of LacZ.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119003/1

Member of these Collections

• iGEM Parts Registry