Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caleb J. Carr
Date created: 2011-08-17 11:00:00
Date modified: 2015-08-31 04:08:26
FINISH
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_J119013_sequence (Version 1) |
Description
Finish half edge for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. BsmBI site pointing right that when digested frees up half edge word "GTGG" on the 5' end that allows ligation to any gene 2 half-edge. Followed by the BsaI site pointing left that when digested frees up the FINISH word "CGAG" that allows ligation to the new tr5f4HPP vector. In between these two sites is an 8 nt spacer. This section is flanked by EcoRI and PstI sticky ends for cloning.Notes
Half-edge words must match the half edge words exactly that you want to ligate to.Source
Oligo constructs designed for the purpose of solving Hamiltonian Path graphs.| Sequence Annotation | Location | Component / Role(s) |
| EcoRI BsmBI 8 NT Spacer Finish Word Half-Edge Word BsaI PstI | 1,6 7,12 17,24 25,28 13,16 29,34 35,40 | sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/dna feature/dna sequence_feature |