BBa_J119014

BBa_J119014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caleb J. Carr

Date created: 2011-08-17 11:00:00

Date modified: 2015-08-31 04:08:26

HPP assembly vector

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_J119014_sequence (Version 1)

Description

New HPP vector for use in Golden Gate Assembly of Hamiltonian Path Problem constructs.
EcoRI site followed by the START sticky word made available by the following BsaI site pointing left. Followed by NheI, then another BsaI site pointing right that frees up the FINISH sticky word. Followed by the PstI site.

Notes

1. Use BsaI to free up a new START word and a new FINISH word that are different from any of the Gene words (listed below).
2. NheI site in the middle for cloning in any XbaI/SpeI BioBrick fragment, such as the ccdB Death Gene or a reporter expression cassette.
3. Flanked by EcoRI and PstI sticky ends.

Source

HPP Vector Oligo designed for use in HPP solutions.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
EcoRI	1,6	sequence_feature, feature/dna
START word	7,10	feature/misc, sequence_feature
BsaI	11,16	sequence_feature, feature/dna
NheI	17,22	feature/dna, sequence_feature
BsaI	23,28	feature/dna, sequence_feature
FINISH word	29,32	feature/misc, sequence_feature
PstI	33,38	feature/dna, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119014/1

Member of these Collections

• iGEM Parts Registry