Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2012-01-02 12:00:00
Date modified: 2015-08-31 04:08:26
START(pTet)-GFP1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J119038_sequence (Version 1) |
Description
Half-edge for use in assembly of Hamiltonian Path Problem graphs. Composed of START half-edge and GFP1 half-edge. Contains START word and GFP gene-specific word that become available for ligation upon digestion with BsaI during Golden Gate Assembly.Notes
Contains START word and GFP gene-specific word that become available for ligation upon digestion with BsaI during Golden Gate Assembly.Source
Ligation of cloned parts.| Sequence Annotation | Location | Component / Role(s) |
| EcoRI BsaI forward START word pTet B0034 GFP1 GFP word PstI BsaI reverse | 1,6 7,12 14,17 18,71 78,89 98,456 449,452 459,465 454,459 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |