BBa_J119061

BBa_J119061 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119061

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl

Date created: 2012-01-27 12:00:00

Date modified: 2015-08-31 04:08:26

pSB1A2-BR

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_J119061_sequence (Version 1)

Description

Plasmid pSB1A2 with BsaI site removed from Ampicillin resistance gene. For use in Golden Gate Assembly with BsaI.

Notes

Original amino acid sequence of Amplicillin gene retained.

Source

PCR mutagenesis of pSB1A2.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Suffix	1,21	feature/BioBrick, engineered_region
Prefix	2058,2079	feature/BioBrick, engineered_region
VR	79,98	feature/primer_binding, primer_binding_site
VF2	1940,1959	feature/primer_binding, primer_binding_site
rep (pMB1) (rev)	183,797	CDS, feature/cds
Ampicillin resistance gene (rev)	957,1817	feature/cds, CDS
Terminator	2029,2048	feature/stem_loop, stem_loop
c -> a (BsaI site removed)	1101,1101	point_mutation, feature/s_mutation

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119061/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors