Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Carr
Date created: 2012-03-09 12:00:00
Date modified: 2015-08-31 04:08:27
Lac-Z Node for GGA/HPP 2.0
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_J119085_sequence (Version 1) |
Description
This part is one of several similar parts designed in our lab to serve as "Nodes" in a Hamiltonian Path Problem experiment using Golden Gate Assembly. This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a Lac-Z reporter.Notes
This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a Lac-Z reporter.Source
de novo| Sequence Annotation | Location | Component / Role(s) |
| EcoRI Word EcoRI cuts left BbsI cuts right BbsI word (Node Word) Lac-Z Node (2nd Codon) Exit Word (Lac-Z) BsaI cuts left PstI cuts right | 1,4 5,10 11,16 18,21 22,1605 1606,1609 1611,1616 1617,1622 | feature/dna sequence_feature sequence_feature feature/misc feature/misc sequence_feature feature/dna sequence_feature CDS feature/protein feature/dna sequence_feature feature/misc sequence_feature feature/misc sequence_feature |