Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hannah Huff, Andie Cassity
Date created: 2012-03-27 11:00:00
Date modified: 2015-08-31 04:08:27
UV promoter w/ RBS and lacZ gene
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J119089_sequence (Version 1) |
Description
This part consists of a UV promoter attached to the lacZ gene with a RBS in front of it. The promoter is activated by ultra-violet light to transcribe the lacZ gene. When plated with X-gal, the part should function as a reporter for exposure to UV light by the mechanism of lacZ cleaving X-gal to turn blue.Notes
When ligating the sub-parts together, the plasmid containing the UV promoter opened and the lacZ w/ RBS was inserted. This is due to the small size of the UV promoter. It should be noted that the UV sub-part was found to be slightly larger than reported on the registry.Source
Existing Registry parts.| Access | Instance | Definition |
| public public | BBa_I765001 BBa_I732017 | pUV BBa_I732017 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I765001 BBa_I732017 | 1,76 85,3177 | pUV BBa_I732017 |