Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brandon Grieshaber and Todd Eckdahl
Date created: 2013-07-10 11:00:00
Date modified: 2015-08-31 04:08:28
pSB1A2-BR-P5-RNA-I
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J119311_sequence (Version 1) |
Description
This vector is pSB1A2 with its BsaI site removed (BR = BsaI Removed), so it can be used in BsaI Golden Gate Assembly. It also has a P5 promoter (BBa_J119031) in place of the natural promoter for RNA I in the pMB1 origin of replication.Notes
Introduction of the P5 promoter mutation was performed for the purpose of changing the plasmid copy number.Source
Junction Golden Gate Assembly using BsaI, T4 DNA Ligase, inverted PCR of pSB1A2-BR and annealed oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| Suffix rep (pMB1) (rev) P5 promoter APr (rev) terminator Prefix | 1,21 183,784 599,634 944,1803 2016,2035 2045,2066 | engineered_region feature/BioBrick CDS feature/cds feature/promoter promoter feature/cds CDS feature/stem_loop stem_loop engineered_region feature/BioBrick |