BBa_J119330

BBa_J119330 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119330
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-01-13 12:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Fitness Module with Riboswitch + ThyA



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_J119330_sequence (Version 1)

Description

The P5 promoter drives a riboswitch (M6C) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a ThyA (thymidylate synthase) fitness gene.

Notes

None.

Source

Golden Gate Assembly with BsaI of J119137 and PCR product of ThyA.

Sequence Annotation Location Component / Role(s)
ThyA
Ammeline Riboswitch
P5 promoter
183,980
73,188
19,55
feature/cds CDS
feature/rbs ribosome_entry_site
promoter feature/promoter
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119330/1