BBa_J119339

BBa_J119339 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119339
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-02-07 12:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Fitness Module with Riboswitch M6 prime + ThyA



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_J119339_sequence (Version 1)

Description

The P5 promoter drives a riboswitch (M6 prime from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a ThyA (thymidylate synthase) fitness gene.

Notes

None.

Source

Mutagenesis of BBa_J119330 with inverse PCR followed by BsaI Golden Gate Assembly.

Sequence Annotation Location Component / Role(s)
P5 promoter
M6 prime Ammeline riboswitch
ThyA
19,55
73,188
183,980
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119339/1