BBa_J119343

BBa_J119343 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119343
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-02-14 12:00:00
Date modified: 2015-08-31 04:08:28

Ammeline Biosensor with Riboswitch M6C prime



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_J119343_sequence (Version 1)

Description

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C prime from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.

Notes

None.

Source

Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly.

Sequence Annotation Location Component / Role(s)
M6C prime Ammeline riboswitch
P5 Promoter
RFP
73,188
19,55
183,866
feature/rbs ribosome_entry_site
feature/promoter promoter
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119343/1