Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kelly Cochran, Mike Quaney
Date created: 2014-07-21 11:00:00
Date modified: 2015-08-31 04:08:28
Ammeline Fitness Module with Riboswitch N2 + TetA (T85A, T89A, G93C, T94C, G95T, T96G)
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_J119363_sequence (Version 1) |
Description
The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.Notes
N2, TetASource
N2, TetA| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Ammeline Riboswitch TetA T to A mutation T to A mutation G to C mutation T to C mutation G to T mutation T to G mutation | 19,55 73,190 185,1382 85,85 89,89 93,93 94,94 95,95 96,96 | promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS feature/mutation sequence_alteration feature/mutation sequence_alteration sequence_alteration feature/mutation feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration |