Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Fall 2014 Tuesday 2:00 lab
Date created: 2014-11-07 12:00:00
Date modified: 2015-08-31 04:08:28
Ptac mutant 15 A's inserted after -35 region
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119371_sequence (Version 1) |
Description
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2014. It was cloned into the pClone Red vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation inserts 15 As immediately after the -35 region. The mutation had a large effect on the function of the promoter (see Experience page).Notes
Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.Source
Synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| 15 As inserted -10 element -35 region | 13,27 44,49 7,12 | sequence_alteration feature/mutation promoter feature/promoter feature/promoter promoter |