BBa_J119393

BBa_J119393 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J119393
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony J. Eckdahl
Date created: 2016-04-28 11:00:00
Date modified: 2016-04-29 10:35:53

Collection of Bicistronic RBSs from N8 rClone Red Library



Types
DnaRegion

Roles
ribosome_entry_site

RBS

Sequences BBa_J119393_sequence (Version 1)

Description

Oligonucleotides that encoded Bicistronic RBSs of the form NNNGANNN were cloned with BsaI Golden Gate Assembly into rClone Red (BBa_J119384). to produce a library of 65,536 possibilities. Clones were picked from the library and sequenced. The RBS strength of each RBS clone was measured with fluorometry and is expressed as a percentage of the strongest clone found in the project, which is a bicistronic C dog RBS. Below is a table of the RBS sequences and strengths, and a consensus sequence that takes into account every base at every position and the relative RBS strengths.

Notes

The oligos needed to include a 5' sticky end of CGAC and a 3'sticky end of CCGC to ligate into rClone Red with BsaI Golden Gate Assembly.

Source

Synthetic oligos cloned into rClone Red (BBa_J119384).

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J119393/1