BBa_J119401

BBa_J119401 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J119401

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Avery Umphreys

Date created: 2016-07-17 11:00:00

Date modified: 2016-07-18 09:19:51

tCloneTet+PDB riboswitch with P5 and BD2

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_J119401_sequence (Version 1)

Description

The BD2 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD2 was inserted via Decoupled Golden Gate Assembly.

Notes

BD2 was inserted to the plasmid via Decoupled Golden Gate Assembly. Using iPCR, all of the J100255 plasmid was amplified, besides BD18.

Source

J100255, original tClone tet+PDB Riboswitch, is located in Campbell M Lab. BD2, located in Eckdahl Lab, is part number J119025.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BD2	225,308	ribosome_entry_site, feature/rbs
p5	105,140	feature/promoter, promoter
TetA	310,1243	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J119401/1

Member of these Collections

• iGEM Parts Registry