BBa_J133002

BBa_J133002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J133002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Norman Wang

Date created: 2010-12-17 12:00:00

Date modified: 2015-08-31 04:08:30

GFPFrag+OligoN

Details

• Types: DnaRegion
• Roles: sequence_feature; Temporary
• Sequences: BBa_J133002_sequence (Version 1)

Description

aka GFPFrag + OligoN
Merger of 2 Parts:
1.) PCRed B0034+E0040 5' fragment from E0240 with mutagenesis primers to remove BsmBI
2.) 326bp Synthetic fragment (OligoN) to include:
a.) 3' fragment of E0040, removing BsaI and
b.) RNAseE+NheI+NsiI+RNAseE+Hairpin (reverse engineered from pSB1A10)

Notes

gene synthesis from IDTDNA, $220 (max 400bp), ordered on 9/22/10, arrived 10/27/10

Source

Source DNA:
backbone: PCRed using G1000-G1001 from pSB3K5-J732720
insert1: PCRed using custom mut primers g0001_f-g0002_r from E0240 E Coli cryostock
insert2: insert freed from pIDTSMART-OligoN plasmid prep

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J133002/1

Member of these Collections

• iGEM Parts Registry