BBa_J18945

BBa_J18945 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18945
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36

ZipR34 ~ GLucFrag2



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_J18945_sequence (Version 1)

Description

P13 expression cassette
from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture:

  • T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop


Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts.


Expression



Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity.

Reference



  • Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices


Notes

Constructs were assembled according to R.F.C. 25.

All parts were codon optimized for E. coli.

Source

synthetic

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J18945/1