| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_J212006_sequence (Version 1)
|
Description
A novel GH5 cellulase gene exo2b was identified from a metagenomic library. The fragment encoding the Exo2b catalytic domain was then fused with the cbh1 gene from Trichoderma reesei, and the fused gene was successfully expressed in T. reesei Rut-C30 .Compared to that of the parent strain, the filter paper activity (FPA) and CMCase activity of the secreted proteins of a selected transformant A1, increased 24% and 18%, respectively.
Notes
The foreign gene exo2b wasfused with the native gene cbh1 to facilitate the translocation of the foreign protein in the secretory pathway of Trichoderma reesei and avoid degradatio
Source
Metagenomic fosmid library of a biogas digester fed with pig manure and rice straw
