BBa_J23004

BBa_J23004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J23004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-06-22 11:00:00

Date modified: 2015-08-31 04:08:38

Plasmid for destroying biobrick restriction sites

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_J23004_sequence (Version 1)

Description

This plasmid is used to destroy the XbaI or SpeI restriction sites of a biobricked part.

Inserting an XbaI/PstI fragment of a biobrick part into the SpeI/PstI sites of pSB*dXS destroys the XbaI site resulting in a plasmid named pSB*dX-[part_name].

Inserting an EcoRI/SpeI fragment of a biobrick part into the EcoRI/XbaI sites of pSB*dXS destroys the SpeI site resulting in a plasmid named pSB*dS-[part_name].

To construct restiction-site deficient variants of a part in a different pSB* plasmid, transfer the EcoRI/PstI fragment into similar sites.

Notes

N/A

Source

PCR ca1002F/R on pSB1A2-I13522 (991 bp, EcoRI/PstI)
Sub into pSB1A2
Product is pSB1A2dXS

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ca1002F Forward EcoRI oligo to make XbaI blunter cassette
cttctggaattcgcggccgcaactagtgtccctatcag
ca1002R Reverse PstI oligo to make SpeI blunter cassette
gaagcctgcagcggccgctTctagAatataaacgcag

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
OnRFP	33,955	feature/misc, sequence_feature
ColE1 origin	1213,1895	sequence_feature, feature/misc
Bla	1993,2652	CDS, feature/cds

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J23004/1

Member of these Collections

• iGEM Parts Registry