BBa_J36845

BBa_J36845 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J36845
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-30 12:00:00
Date modified: 2015-08-31 04:08:47

Lpp-OmpA(46-66)-Streptavidin single-chain dimeric + His6



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_J36845_sequence (Version 1)

Description

Assembled construct of signal peptide of lipoprotein, one transmembrane domain of outer membrane protein A, and streptavidin, single-chain dimeric with His6 tag at the end.

This construct codes for a protein which displays streptavidin on the cell surface.

Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.

Source

Assembly of J36835, J36836, J36843

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J36845/1