BBa_J36847

BBa_J36847 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J36847

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Perry Tsai

Date created: 2006-10-30 12:00:00

Date modified: 2015-08-31 04:08:47

Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_J36847_sequence (Version 1)

Description

Assembled construct of signal peptide of lipoprotein, five transmembranes domain of outer membrane protein A, and streptavidin, single-chain dimeric with His6 tag at the end.

This construct codes for a protein which displays streptavidin on the cell surface.

Notes

There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.

Source

Assembly of J36835, J36837, J36843

Components

Access	Instance	Definition
public	BBa_J36835	BBa_J36835
public	BBa_J36837	BBa_J36837
public	BBa_J36843	BBa_J36843

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J36835	1,87	BBa_J36835
BBa_J36837	96,437	BBa_J36837
BBa_J36843	446,1264	BBa_J36843

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J36847/1

Member of these Collections

• iGEM Parts Registry