BBa_J44012

BBa_J44012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J44012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Todd Eckdahl
Date created: 2006-10-25 11:00:00
Date modified: 2015-08-31 04:08:49

HixC-pBAD-HixC-RBS-TetF-HixC



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_J44012_sequence (Version 1)

Description

Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from forward to reverse orientation. Cloned in pSB1A7 - readthrough transcription not detected.

Notes

Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.

Source

Cloned from BBa_J44010

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J44012/1